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KMID : 0603520000050030117
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Journal of Korean Association of Cancer Prevention
2000 Volume.5 No. 3 p.117 ~ p.125
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Antimutagenic and Mutagenic Test in Big Blue® Transgenic Mouse Embryonic Cell
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Eom Mi-Ok
Han Eui-Sik
Kim Jong-Won
Sohn Soo-Jung
Ryu Byung-Kyu
Byun Eun-Kyung
Choi Jae-Sue
Oh Hye-Young
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Abstract
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Transgenic animal cell with a λshuttle vector containing a lac I target gene is useful as a short-term in vitro mutagenesis assay. The gene is recovered from the treated cell by exposing mouse genomic DNA to in vitro packaging extracts and plating the rescued phage on agar plates containing 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal). Phage with mutations in the lac I gene form blue plaques, whereas phage with a nonmutated lac I form colorless plaques. In order to estabilish this new transgenic Technology, we studied the mutagenicity of the direct-acting alkylating agent and indirect mutagen benzo[a]pyrene (B[a]P) in the Big Blue® transgenic mouse embryonic cell line. MNU or B[a]P caused an induction of mutations over negative control, 1% DMSO and we confirmed the reproducibility of the transgenic mutagenicity assay. Also, dose- dependent response has been observed in cells treated with B[a]P. When the antimutagenic effect of Ecklonia strolonifera, an edible brown algae having a strong antioxidizing activity, on MNU-induced mutation was evaluated, the methanol extract of Ecklonia strolonifera showed reduction of mutant frequency in cells treated with MNU.
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KEYWORD
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Transgenic mouse embryoonic cell, N-methyl-N-nitrosourea (MNU), Benzo[a]pyrene (B[a]P), Ecklonia stolonifera, Mutation assay, Anti-mutagenicity
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